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High-speed cell sorting (staff or self-operated, AriaII-3) They are also encouraged to initially sort during working hours when FACS staff are present until they have acquired experience with troubleshooting and problems that may arise during sorting. People are encouraged to attend the two training sessions in a short time frame between each other. Training consists of two sessions in which modalities of sorting and instrument set ups are illustrated (see instructions and training section). Trained operators will be able to reserve time directly on the AriaII-2 schedule. High-speed cell sorting (self-operated, AriaII-2) changes in nozzle size, changes in pressure, single cell sorting into 96- or 384- well plates), please include this information in the notes section of the sorting request form and add 30 minutes to your requested sorting time to allow changes from the default setup (NOTE: you will be charged for this special setup time). If your sort requires a special setup (i.e. If the setup notes for your sorting do not indicate special requirements, the AriaII-1 will be set up with a default 85um nozzle 45psi. If you do not receive a confirmation email, your sort has not been scheduled. You will receive a confirmation email when your request has been added to the schedule.
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An email will be sent to the FACS staff detailing your request and your request will be added to the sorting calendar. To schedule cell sorting on the Aria II-1, go to the online schedule, choose the time you would like to begin and end, click on REQUEST and fill out the pop-up window with your lab and sort information. Requests for cell sorting can be made using the online schedule or by contacting the FACS Staff by email at The online schedule is accessible only to Washington University researchers and requires a username and password.
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High-speed cell sorting (staff-operated, AriaII-1)Īppointments with staff are available Monday through Friday from 10am to 5pm. Please note that each user needs to have an individual account. First time users: please register here. When you log in fill in all required information and request access to instruments. Please allow one business day for the activation of your account. Contact the FACS Staff if you have any questions or problems with the new account at and pricing Service available to: All entities, including for-profit organizations.
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Fixed an issue where the selected parameter in the Layout Editor graph plot where the Y axis was not displaying the full name of the parameter.Fixed a problem where keyboard shortcuts would not work in numeric text fields.Fixed an issue in Population Comparison where the bin setting would be lost when the platform was closed.Fixed an issue where XML Excel table export did not contain a header row.Improved the default scaling of Yeti data.Fixed an issue where we were erroneously filtering out height parameters in some cases.Found and fixed an error in combination gates applied (dragged) to sibling populations.Fixed a rare issue where some data files would shows No $PnS Keywords/Labels.Fixed an issue saving the Miltenyi Transform in Customize Axis Dialog.Fixed a rare issue with Mo-Flo Astrios Files regarding the parameter filter string.Fixed an issue where Layout overlays reverted to default properties when changes to legend were applied.Better handling of fcs files with null entries in matrix file.(A side effect of this is that you will see a dialog asking if you want to save a workspace even though it hasn’t changed) Fixed an issue where very large and complex workspaces could take a very long time to close.Improved the file saving behavior with ACS files.